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Registros recuperados: 68
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Mitochondrial and nuclear DNA phylogeography of two cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf
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Phylogenetic relationships of the European newts (genus Triturus) tested with mitochondrial DNA sequence data Naturalis
Zajc, I.; Arntzen, J.W..
European newts (genus Triturus) are widely studied, but their phylogeny is not yet unambiguously resolved. Fragments of mitochondrial DNA experiencing different rates of evolution (the ATPase and 12S rRNA genes) were sequenced in order to test a phylogenetic hypothesis derived from biochemical and behavioral data. Well-supported branches of the existing phylogeny gained support in our study. The monophyletic origin of the hypothesized T. boscai — T. italicus clade remained ambiguous, whereas strong support was gained for the sister-taxon relationship of T. vulgaris and T. montandoni. The position of T. vittatus as a sister taxon to the T. marmoratus species group was also supported. The phylogenetic position of T. alpestris could not be clarified. With an...
Tipo: Article / Letter to the editor Palavras-chave: 12S rRNA; ATPase; Mitochondrial DNA; Newt; Phylogeny; Triturus.
Ano: 1999 URL: http://www.repository.naturalis.nl/record/534335
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Hierarchical analysis of mtDNA variation and the use of mtDNA for isopod (Crustacea: Peracarida: Isopoda) systematics Naturalis
Wetzer, R..
Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S, 16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent...
Tipo: Article / Letter to the editor Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI.
Ano: 2001 URL: http://www.repository.naturalis.nl/record/534397
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Muscle cell outside and inside: the nascent approach of a clinician Biol. Res.
FADIC,RICARDO.
Understanding muscle cell in disease and health is an unfinished process. Following the lead of Jaime Alvarez, I have had the opportunity of working on two complementary approaches to this field. One is the study of muscle cell surface molecules. Both synaptic muscle molecules, such as the asymmetric form of acetylcholinesterase, and extrasynaptic molecules, such as the extracellular matrix proteoglycans, are regulated by the motor nerve activity. This illustrates one of Jaime's teachings: cell phenotypes are a dynamic process that reflects the influence of other cells (<A HREF="#0">Alvarez, 2001</A>). Proteoglycans have many functions, including growth factor receptors. Studying them in muscular dystrophy will contribute to the comprehension...
Tipo: Journal article Palavras-chave: Mitochondrial DNA; Muscle disease; Muscular dystrophy; Proteoglycans.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200018
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Variation in the mitochondrial control region sequence between the two maternal lines of the Sorraia horse breed Genet. Mol. Biol.
Luís,Cristina; Bastos-Silveira,Cristiane; Cothran,E.Gus; Oom,M. Mar.
The mitochondrial control region sequence was compared between the two surviving matrilineal lineages of the Sorraia horse. Two haplotypes were found, showing three differences between them. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorraia horse; Mitochondrial DNA; Maternal lineages; Control region.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300010
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Mitochondrial DNA phylogeography of Brazilian populations of Drosophila buzzatii Genet. Mol. Biol.
Brito,Reinaldo A. de; Manfrin,Maura H.; Sene,Fabio M..
The Argentinean Chaco region has been considered the center of origin of Drosophila buzzatii in South America because it contains most of the chromosomal polymorphism detected in natural populations. Two hypotheses have been put forward to explain the distribution of D. buzzatii in Brazil, one proposing that it has only recently passively colonized Brazil via human dispersal and the other suggesting that D. buzzatii has actively migrated to Brazil some time ago. Data from chromosomal inversions support recent colonization, whereas data from allozymes and mtDNA variation indicate that D. buzzatii has been in Brazil longer, favoring an active dispersal hypothesis. In our present work we analyzed data on 56 South American flies, mostly from Brazil, sequenced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila buzzatii; Population structure; Mitochondrial DNA; Phylogeography; Brazil; Climatic cycles.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200009
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Identification of Astyanax altiparanae (Teleostei, Characidae) in the Iguaçu River, Brazil, based on mitochondrial DNA and RAPD markers Genet. Mol. Biol.
Prioli,Sônia M.A.P.; Prioli,Alberto J.; Júlio Jr.,Horácio F.; Pavanelli,Carla S.; Oliveira,Alessandra V. de; Carrer,Helaine; Carraro,Dirce M.; Prioli,Laudenir M..
Astyanax fishes are among the most important food-web components of South America rivers. In the Iguaçu River basin, the Astyanax genus is represented mainly by endemic species. For millions of years, that hydrographic basin has been geographically isolated from the Paraná River basin by the Iguaçu Falls. Recently, a species from the Upper Paraná River basin identified as Astyanax bimaculatus was revised and described as a new species named Astyanax altiparanae Garutti &amp; Britski, 2000. Fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two A. altiparanae populations from the Upper Paraná River basin and the population identified as A. bimaculatus in the Iguaçu...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax; Fish; D-loop; Control region; Mitochondrial DNA; RAPD.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400011
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Lineage Classification of Canine Inheritable Disorders Using Mitochondrial DNA Haplotypes OAK
TAKAHASI, Shoko; MIYAHARA, Kazuro; ISHIKAWA, Hirosi; ISHIGURO, Naotaka; SUZUKI, Mitsuyoshi; 宮原, 和郎; 鈴木, 三義.
To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of...
Palavras-chave: D-loop; Haplotype lineage analysis; Mitochondrial DNA.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4066
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Phylogenetic analysis of 16S mitochondrial DNA data in sloths and anteaters Genet. Mol. Biol.
Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio.
We sequenced part of the 16S rRNA mitochondrial gene in 17 extant taxa of Pilosa (sloths and anteaters) and used these sequences along with GenBank sequences of both extant and extinct sloths to perform phylogenetic analysis based on parsimony, maximum-likelihood and Bayesian methods. By increasing the taxa density for anteaters and sloths we were able to clarify some points of the Pilosa phylogenetic tree. Our mitochondrial 16S results show Bradypodidae as a monophyletic and robustly supported clade in all the analysis. However, the Pleistocene fossil Mylodon darwinii does not group significantly to either Bradypodidae or Megalonychidae which indicates that trichotomy best represents the relationship between the families Mylodontidae, Bradypodidae and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pilosa; Sloths; Anteaters; Phylogeny; Mitochondrial DNA; RDNA16S.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100002
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Mitochondrial Genetic Characterization Of Bluefin Tuna (Thunnus Thynnus) From Three Mediterranean (Libya, Malta, Tunisia); And One Atlantic Locations (Gulf Of Cadiz). OceanDocs
Farrugia, A.F.; Pla, C.; Viñas, J.; Tawil, M.Y.; Hattour, A.; de la Serna, J.M..
None of the previous genetic analysis found significant differences within the Mediterranean. However, a more exhaustive analysis is needed inside this sea. In this sense, we have analyzed the mitochondrial control region sequence variability of three Mediterranean locations: Libya (n = 22), Tunisia (n = 23) and Malta (n = 12). Additionally a sample from the Gulf of Cadiz (n = 24) in the East Atlantic Ocean was also included in the study. The AMOVA analysis revealed slightly (Fst = 0.013) but significative genetic differences (P < 0.000) for the Malta location, probably due to its small sample size (n = 12). In the other hand, when this location is removed from the analysis, no differences where found among the three remaining location (Fst = 0,005; P =...
Tipo: Journal Contribution Palavras-chave: Atlantic ocean; Mitochondrial DNA; Bluefin tuna; Sequencing; Genetic characterization; Mediterranean sea; Tuna fisheries.
Ano: 2003 URL: http://hdl.handle.net/1834/792
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Sciaenidae fish of the Caeté River estuary, Northern Brazil: mitochondrial DNA suggests explosive radiation for the Western Atlantic assemblage Genet. Mol. Biol.
Vinson,Christina; Gomes,Grazielle; Schneider,Horacio; Sampaio,Iracilda.
Sciaenids are fish which are normally abundant in tropical estuaries of the western Atlantic. Studies on the Caeté river estuary in the northern Brazilian state of Pará have revealed that in this area Sciaenidae is the dominant family, comprising almost 50% of all teleosts sampled. In this paper we present the results of the first phylogenetic study on South American estuarine sciaenids, during which we obtained mitochondrial gene 16S sequences from 15 species belonging to eight genera occurring in the Caeté estuary. Intergeneric nucleotide divergences varied from 5 to 15%, Lonchurus and Menticirrhus being the most divergent lineages. Nucleotide divergences were quite variable amongst species of the same genus, ranging from 1.2% (Stellifer microps x...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sciaenidae; Perciformes; Mitochondrial DNA; RRNA 16S; Caeté River estuary.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200008
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The value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera: Calliphoridae) of potential forensic importance Neotropical Entomology
Thyssen,Patricia J.; Lessinger,Ana C.; Azeredo-Espin,Ana M.L.; Linhares,Arício X..
The identification of insect species involved in corpses decomposition is of particular importance in estimating the post-mortem interval (PMI) in forensic science, since the PMI is based on information about the life cycle of necrophagous insects. However, the identification of some insect species, especially in their immature stages, may be complicated by many factors, even for experienced taxonomists. Species of the same genus such as Hemilucilia segmentaria (Fabricius) and H. semidiaphana (Rondani) (Diptera: Calliphoridae) are morphologically and behaviorally very similar, but differ in their growth and maturation rates. These species are abundant in forests, exclusively necrophagous and, therefore, are of potential medicolegal importance for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forensic entomology; Mitochondrial DNA; Hemilucilia segmentaria; Hemilucilia semidiaphana.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000500009
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Demographic history of wood stork (Mycteria americana) Brazilian Pantanal colonies revealed by mitochondrial DNA analysis Genet. Mol. Biol.
Lopes,Iara Freitas; Brito,Reinaldo Alves de; Henrique-Silva,Flávio; Del Lama,Silvia Nassif.
We used mitochondrial DNA (mtDNA) sequences to investigate the demographic history of the wood stork (Mycteria americana) populations in the Brazilian Pantanal. Sequences of 390/460 bp fragment of the mtDNA control region were analyzed in 62 wood stork specimens from 8 colonies using neutrality tests, phylogeographic, and coalescent analyses. Population expansion was supported by the significantly negative values of Tajima’s (D = -2.071) and Fu’s (Fs = -14.544) statistics and the unimodal pattern of mismatch distribution. Nested clade analyses indicated a historic range expansion event and recurrent gene flow that was restricted by isolation by distance as explanations for the haplotype distribution among the sampled colonies. High genetic diversity and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wood stork; Mycteria americana; Pantanal; Mitochondrial DNA; Phylogeography.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200008
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A recombination point is conserved in the mitochondrial genome of higher plant species and located downstream from the cox2 pseudogene in Solanum tuberosum L. Genet. Mol. Biol.
Tada,Susely F.S.; Souza,Anete P..
The potato (Solanum tuberosum L.) mitochondrial cox3/sdh4/pseudo-cox2 gene cluster has previously been identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In our present study we used Southern blotting using sdh4 and cox2 probes to show that the sdh4 and cox2 genes are clustered in the mitochondria of potato, soybean and pea. Northern blotting revealed cotranscription of sdh4 and cox2 in potato but not in cauliflower, indicating that these genes are not clustered in cauliflower. A putative recombination point was detected downstream of the cox2 pseudogene (pseudo-cox2) in potato mitochondrial DNA (mtDNA). This sequence corresponds to a 32 bp sequence which appears to be well-conserved and is adjacent to the terminals of some...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondrial DNA; Rearrangement; Plants; Solanum tuberosum; Cox2.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100017
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Cytogenetic and molecular analysis of the Pantaneiro cattle breed PAB
Issa,Érica Cunha; Jorge,Wilham; Sereno,José Robson Bezerra.
The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos taurus taurus; Bos taurus indicus; Y chromosome; Mitochondrial DNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100006
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Genetic structure of Brazilian populations of white mouth croaker Micropogonias furnieri (Perciformes : Sciaenidae) BABT
Puchnick-Legat,Angela; Levy,José Alberto.
Mitochondrial (mt) DNA population structure was examined by using restriction fragment length polymorphism analysis (RFLP) among 149 white mouth croakers (Micropogonias furnieri) sampled from six locations in the Brazilian coast. Heterogeneity tests revealed no differentiation in mtDNA haplotype frequencies within the region between 23°S and 34°S (P=0.263), but significant heterogeneity was detected between north and south of 23°S (P<0.003). Analysis of molecular variance (AMOVA) indicated a low F ST value (0.008, P=0.180) among south-central localities (23-34°S), but a significant level of population subdivision (F ST = 0.322, P< 0.003) between north and south-central localities. UPGMA analysis of mtDNA sequence divergence revealed differentiation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic population structure; Mitochondrial DNA; RFLP; Micropogonias furnieri.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400011
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Prevalence of the A1555G (12S rRNA) and tRNA Ser(UCN) mitochondrial mutations in hearing-impaired Brazilian patients BJMBR
Abreu-Silva,R.S.; Lezirovitz,K.; Braga,M.C.C.; Spinelli,M.; Pirana,S.; Della-Rosa,V.A.; Otto,P.A.; Mingroni-Netto,R.C..
Mitochondrial mutations are responsible for at least 1% of the cases of hereditary deafness, but the contribution of each mutation has not yet been defined in African-derived or native American genetic backgrounds. A total of 203 unselected hearing-impaired patients were screened for the presence of the mitochondrial mutation A1555G in the 12S rRNA gene and mutations in the tRNA Ser(UCN) gene in order to assess their frequency in the ethnically admixed Brazilian population. We found four individuals with A1555G mutation (2%), which is a frequency similar to those reported for European-derived populations in unselected samples. On the other hand, complete sequencing of the tRNA Ser(UCN) did not reveal reported pathogenic substitutions, namely A7445G,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondrial DNA; Hearing impairment; A1555G mutation; TRNA Ser(UCN) mutations; European- and African- Brazilian patients.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200008
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Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves) Genet. Mol. Biol.
Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W..
Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029
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Diversité et différentiation génétiques des populations de tortues vertes (Chelonia mydas) dans les sites de ponte et d'alimentation du sud-ouest de l'océan Indien : application aux stratégies de conservation de l'espèce ArchiMer
Taquet, Coralie.
The green turtle (Chelonia mydas) is an emblematic species of marine life. However, nowadays it is subject to many threats (poaching, by-catch). Even if there is deep growing measures for its protection, the green turtle still is an endangered species and it is listed in Appendix I of Washington Convention (CITES). In order to elaborate efficient conservation and management plans, perfect knowledge of green turtle biology, but also of its population structure and their characteristics, are needed. In this thesis, we have assessed genetic structure of green turtle populations in the South-Western Indian Ocean by using genetic tools. In all, 1551 tissue samples have been collected from our study zone and from our control site French Polynesia (37 samples)....
Tipo: Text Palavras-chave: Management units; Microsatellites; Mitochondrial DNA; Foraging sites; Nesting sites; South Western Indian Ocean; Population genetics; Chelonia mydas; Green turtle; Unités de gestion (MUs); Microsatellites; ADN mitochondrial; Sites d'alimentation; Sites de ponte; Génétique des populations; Chelonia mydas; Tortue verte.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-3532.pdf
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Phylogeography of the green turtle, Chelonia mydas, in the Southwest Indian Ocean ArchiMer
Bourjea, Jerome; Lapegue, Sylvie; Gagnevin, L; Broderick, D; Mortimer, J.a.; Ciccione, S; Roos, David; Taquet, Coralie; Grizel, Henri.
Patterns of mitochondrial DNA (mtDNA) variation were used to analyse the population genetic structure of southwestern Indian Ocean green turtle (Chelonia mydas) populations. Analysis of sequence variation over 396 bp of the mtDNA control region revealed seven haplotypes among 288 individuals from 10 nesting sites in the Southwest Indian Ocean. This is the first time that Atlantic Ocean haplotypes have been recorded among any Indo-Pacific nesting populations. Previous studies indicated that the Cape of Good Hope was a major biogeographical barrier between the Atlantic and Indian Oceans because evidence for gene flow in the last 1.5 million years has yet to emerge. This study, by sampling localities adjacent to this barrier, demonstrates that recent gene...
Tipo: Text Palavras-chave: Phylogeography; Mozambique Channel; Mitochondrial DNA; Indian Ocean; Control region; Chelonia mydas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2185.pdf
Registros recuperados: 68
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